pdquest 2de analysis software  (Bio-Rad)

Journal: Advances in Cancer Biology - Metastasis

Article Title: Systemic aftermaths of tobacco addiction on the physiological composite of human secretome and the prognostic potential of U2AF26 in oral cancers

doi: 10.1016/j.adcanc.2022.100075

Figure Lengend Snippet: Fig. 2. MALDI TOF MS PMF map and database query result of 2DE protein spot ‘a’, that was identified as Homo sapiens ‘splicing factor U2AF 26 kDa (U2AF26)’. (A) MS map of spot ‘a’ with matched peptide peaks labeled with their mass value. (B) Peptide mass fingerprint matched (protein score 77) with HCUP and the probability of a random match event was <0.05. (C) Given are the mass analysis results between the observed peptide sequence and HCUP sequence. The ‘start-end’ represents the amino acid locus in the protein sequence; the ‘observed’ represents the observed mass; ‘Mr (expt)’ represents the expected mass; ‘Mr (calc)’ represents the calculated theoretical mass. (D) NCBI BLAST result (matched peptide sequences highlighted in green); the observed peptides showed 37% sequence coverage with U2AF26. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The gels were compared by PDQuest 2DE analysis software advanced version 8.0.1 (Bio-Rad, USA).

Techniques: Labeling, Sequencing